%0 Journal Article
%T 姜黄素对动脉粥样硬化大鼠血液中炎症因子与血脂的影响及其作用机制
%A 潘海龙　何兴强　成娅　李栋　齐元杰　姚来昱　沃金善
%J -
%D 2020
%R 10.13362/j.jpmed.202002017
%X 摘要 目的　研究姜黄素对动脉粥样硬化（AS）大鼠血液中炎症因子、血脂的影响及其作用机制。 方法　将72只健康SD大鼠随机均分为空白对照组（K组）、模型对照组（M组）、辛伐他汀阳性组（Y组）及姜黄素高、中、低剂量组（G、Z、D组）。采用ELISA法检测各组大鼠血清中白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、血清总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL）含量；HE染色观察各组大鼠主动脉组织病理变化；采用Realtime-PCR法、免疫印迹法检测主动脉组织中磷脂酰肌醇3-激酶（PI3K）、蛋白激酶B（Akt）蛋白及mRNA的表达情况。 结果　单因素方差分析结果显示，6组大鼠血清中IL-6、TNF-α水平比较差异有显著性（F=56.40、65.40，P＜0.01），除M组与D组IL-6差异无显著性外，其他任意两组间差异均有显著性（P＜0.05）；6组血清中TC、TG、LDL水平比较差异有显著性（F=39.66～54.60，P＜0.01），两两比较结果显示除M组与D组、Y组与G组、G组与Z组TC，M组与D组、G组与Z组TG，K组与Y组、G组与Z组LDL差异无统计学意义外，其他组间比较差异均有显著性（P＜0.05）。HE染色结果显示，和K组相比，M组主动脉组织内可见大量炎性细胞、泡沫样细胞浸润及明显增殖的平滑肌细胞，并出现散在钙化灶；Y组中炎性细胞、泡沫样细胞显著减少；G、Z、D组中仍可见炎性细胞、泡沫样细胞浸润及增殖的平滑肌细胞，但同M组相比上述病理改变均得到了显著缓解。6组主动脉组织中PI3K、Akt蛋白和mRNA表达水平比较差异有显著性（F=297.83～2 930.20，P＜0.01），组间比较差异均具有显著性（P＜0.05）。 结论　姜黄素能够显著抑制AS模型大鼠主动脉炎性因子IL-6、TNF-α的生成及降低血清TC、TG、LDL水平，减少主动脉炎性浸润及降低脂质成分的堆积，显示出良好的抗AS效果；并且能够降低主动脉组织中PI3K、Akt蛋白及mRNA的表达，抑制PI3K/Akt信号通路而发挥抗AS作用，该研究为AS治疗药物的研发提供了一定的理论依据。<br>Abstract：Objective　To investigate the effect of curcumin on inflammatory factors and lipids in the blood of atherosclerotic rats and its mechanism of action. Methods　Seventy-two healthy Sprague Dawley rats were randomly divided into blank control group (group K), model control group (group M), simvastatin-positive group (group Y), and high-, medium-, and low-dose curcumin groups (groups G, Z, and D). ELISA was used to determine the serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL) in the rats in each group; HE staining was used to evaluate the pathological changes in the aortic tissue in the rats in each group; the mRNA and protein expression levels of phosphoinositide 3-kinase (PI3K) and protein kinase-B (Akt) in the aortic tissue were determined by real-time PCR and Western blot, respectively. Results　The results of one-way analysis of variance showed that there were significant differences in the serum levels of IL-6 and TNF-α between the six groups (F=56.40,65.40,P<0.01), with significant differences observed in all of the pairwise comparisons except for the comparison of IL-6 between groups M and D (P<0.05); there were significant differences in the serum levels of TC, TG, and LDL between the six groups (F=39.66-54.60,P<0.01), with significant differences observed in all of the pairwise comparisons except for the comparisons of TC between groups M and D, groups Y and G, and groups G and Z, the comparisons of TG between groups M and D and groups G and Z, and the comparisons of LDL between groups K and Y and groups G and Z (P<0.05). HE staining showed that,
%K 姜黄素
%K 动脉粥样硬化
%K 细胞因子类
%K 白细胞介素6
%K 肿瘤坏死因子α
%K 脂类
%K 磷酸肌醇3-激酶
%K 原癌基因蛋白质c-akt
%K 大鼠
%K Sprague-Dawley<br>Curcumin
%K Atherosclerosis
%K Cytokines
%K Interleukin-6
%K Tumor necrosis factor-alpha
%K Lipids
%K Phosphatidylinositol 3-kinase
%K Proto-oncogene proteins c-akt
%K Rats
%K Sprague-Dawley
%U http://jpmed.qdu.edu.cn/CN/10.13362/j.jpmed.202002017