%0 Journal Article %T Transcriptomic Profiling of Yersinia pseudotuberculosis Reveals Reprogramming of the Crp Regulon by Temperature and Uncovers Crp as a Master Regulator of Small RNAs %A Aaron M. Nuss %A Ann Kathrin Heroven %A Barbara Waldmann %A Jan Reinkensmeier %A Michael Beckstette %A Michael Jarek %A Petra Dersch %J - %D 2015 %R 10.1371/journal.pgen.1005087 %X One hallmark of pathogenic yersiniae is their ability to rapidly adjust their life-style and pathogenesis upon host entry. In order to capture the range, magnitude and complexity of the underlying gene control mechanisms we used comparative RNA-seq-based transcriptomic profiling of the enteric pathogen Y. pseudotuberculosis under environmental and infection-relevant conditions. We identified 1151 individual transcription start sites, multiple riboswitch-like RNA elements, and a global set of antisense RNAs and previously unrecognized trans-acting RNAs. Taking advantage of these data, we revealed a temperature-induced and growth phase-dependent reprogramming of a large set of catabolic/energy production genes and uncovered the existence of a thermo-regulated ¡®acetate switch¡¯, which appear to prime the bacteria for growth in the digestive tract. To elucidate the regulatory architecture linking nutritional status to virulence we also refined the CRP regulon. We identified a massive remodelling of the CRP-controlled network in response to temperature and discovered CRP as a transcriptional master regulator of numerous conserved and newly identified non-coding RNAs which participate in this process. This finding highlights a novel level of complexity of the regulatory network in which the concerted action of transcriptional regulators and multiple non-coding RNAs under control of CRP adjusts the control of Yersinia fitness and virulence to the requirements of their environmental and virulent life-styles %K Yersinia pseudotuberculosis %K Antisense RNA %K Non-coding RNA %K Gene expression %K Regulator genes %K Sequence motif analysis %K Virulence factors %K Messenger RNA %U https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005087