%0 Journal Article
%T The Effect of cRGDfV on Behaviour in 3D
Spheroid Invasion Assay and 2D Models
%A Helen Sheldrake
%A Laurance Patterson
%A Manar Zraikat
%A Steve Shnyder
%J -
%D 2017
%X Tumour dissemination is a major reason for failure of therapy for many tumour types therefore there is a requirement for novel targets & therapies. One of the main challenges is having a relevant, validated experimental model that expresses the tumour dissemination process. The aim of the work presented here is to study the effect of the compound cRGDfV on the invasion of U87-MG cell as spheres in 3D model and as single layer in 2D model. cRGDfV inhibited U87- MG spheroid invasion through the gel in a dose and time-dependent manner. However, cRGDfV showed a significantly more potent effect on the invasion of U87-MG cells through Matrigel coated 2D transwells compared to its effect on the invasion of U87-MG cells from spheroids in collagen. The spheroid invasion assay has the advantage over standard monolayer transwell chamber invasion assays of being a 3-dimensional assay, and thus mimics better the cell-cell interactions and architecture that are present in a tumour compared to the monolayer-based assay.
%K Cancer
%K Invasion
%K Migration
%K Spheroids
%K Transwells
%K Pharmacology
%K Metastasis
%K list of open access journals
%K open access
%K open access journals
%K open access publication
%K open access publisher
%K open access publishing
%K open access journal articles
%K imedpub
%K imedpub publishing
%K insight medical publishing
%K imedpub online
%U https://www.imedpub.com/articles/the-effect-of-crgdfv-on-behaviour-in-3dspheroid-invasion-assay-and-2d-models.php?aid=19928