%0 Journal Article
%T <i>In Vitro</i> Culture studies of <i>Bixa orellana</i> L: II- Bixin accumulation in root and hypocotyl derived callus
%A Madhuri Sharon
%A Manisha Sharan
%A Marieclaire Castello
%J -
%D 2012
%X Root and hypocotyl derived calli of Bixa orellana L., cultured on MS medium supplemented with 2 mg/l 2-iP and
  0.05 mg/l NAA showed many localized area having orange pigmentation. The pigments were confirmed to be bixin
  after spectrophotometric analysis. After 2 passages of subculture the pigment accumulation decreased. No further
  continuation of bixin biosynthesis could be induced even after incorporation of various carotenoid precursor viz.
  Geranylgeranylpyrophosphate (50-200 ugl/l GGPP), Mevalonic Acid (50-200 mg/l MA), Gibberellic Acid (0.1- 1
  mg/l GA) or carotenoid biosynthesis cofactor Manganese (10-40 mg/l Mn) into the callusing medium. However,
  GGPP and MA did enhance the accumulated bixin content in the first month.
%K Bixa orellana
%K Bixin
%K Callus
%K Carotene
%K Geranylgeranylpyrophosphate
%K Mevalonic acid
%K Gibberellic
acid
%K 2-isopentenyl adenine (2-iP)
%K list of open access journals
%K open access
%K open access journals
%K open access publication
%K open access publisher
%K open access publishing
%K open access journal articles
%K imedpub
%K imedpub publishing
%K insight medical publishing
%K imedpub online
%U https://www.imedpub.com/articles/iin-vitroi-culture-studies-of-ibixa-orellanai-l-ii-bixin-accumulation-in-root-and-hypocotyl-derived-callus.php?aid=11424