%0 Journal Article
%T Estimation of Sofosbuvir with Validated Ultra High Performance Liquid Chromatographic (UHPLC) Method in its bulk and Formulations
%A Manikanta Kumar A
%A Muniappan M
%A Muralidaran Kaliyaperumal
%A Ramulu Y
%A Shaik John Saida
%J -
%D 2017
%X In this work, a stability indicating and validated UPLC method has been developed for estimation of Sofosbuvir (API) in bulk and its formulations (Sovaldi£¿). The chromatographic separation was achieved on a Waters BEH C18 column (2.1 ¡Á 100 mm, 1.7 ¦Ìm) in an isocratic elution mode with flow rate 0.4 mL/min, the mobile phase of Acetonitrile and Water (30:70) in 0.1% formic acid (pH ~2-3). The optimized method is linear over the concentration range of 20-120 ppm; the Limit of Quantification (LOQ) and Limit of detection are 0.063 and 0.03 ¦Ìg/mL respectively
%K Shaik John Saida*
%K Muniappan M
%K Manikanta Kumar A
%K Muralidaran Kaliyaperumal
%K Ramulu Y and S Venkat Rao*
£¿
%K Sofosbuvir
%K Method development
%K Validation
%K Precision
%K Linearity
%K Regular pharmaceutical analysis
%U https://www.imedpub.com/abstract/estimation-of-sofosbuvir-with-validated-ultra-high-performance-liquid-chromatographic-uhplc-method-in-its-bulk-and-formulations-19629.html