%0 Journal Article
%T Cytisine exerts anti-tumour effects on lung cancer cells by modulating reactive oxygen species-mediated signalling pathways
%A Cheng Wang
%A Cheng-Hao Jin
%A Hao Wang
%A Hui Xue
%A Jia-Ru Wang
%A Long-Kui Cao
%A Shu-Mei Li
%A Tian-Zhu Li
%A Tong Zhang
%A Wan-Ting Xu
%A Xian-Ji Piao
%A Ying-Hua Luo
%A Yu Zhang
%J Artificial Cells, Nanomedicine, and Biotechnology
%D 2020
%R https://doi.org/10.1080/21691401.2019.1699813
%X Abstract Cytisine is a natural product isolated from plants and is a member of the quinolizidine alkaloid family. This study aims to investigate the effect of cytisine in human lung cancer. Cell viability was determined using the CCK-8 assay, and the results showed that cytisine inhibited the growth of lung cancer cell lines. The apoptotic effects were evaluated using flow cytometry, and the results showed that cytisine induced mitochondrial-dependent apoptosis through loss of the mitochondrial membrane potential; increased expression of BAD, cleaved caspase-3, and cleaved-PARP; and decreased expression levels of Bcl-2, pro-caspase-3, and pro-PARP. In addition, cytisine caused G2/M phase cell cycle arrest that was associated with inhibiting the AKT signalling pathway. During apoptosis, cytisine increased the phosphorylation levels of JNK, p38, and I-¦ĘB, and decreased the phosphorylation levels of ERK, STAT3, and NF-¦ĘB. Furthermore, cytisine treatment led to the generation of ROS, and the NAC attenuated cytisine-induced apoptosis. In vivo, cytisine administration significantly inhibited the lung cancer cell xenograft tumorigenesis. In conclusion, cytisine plays a critical role in suppressing the carcinogenesis of lung cancer cells through cell cycle arrest and induction of mitochondria-mediated apoptosis, suggesting that it may be a promising candidate for the treatment of human lung cancer
%U https://www.tandfonline.com/doi/full/10.1080/21691401.2019.1699813