%0 Journal Article
%T FAS-associated factor-1 positively regulates type I interferon response to RNA virus infection by targeting NLRX1
%A Chamilani Nikapitiya
%A Chul-Joong Kim
%A Eunhee Kim
%A Hyun-Cheol Lee
%A Jae U. Jung
%A Jae-Hoon Kim
%A Jin Yeul Ma
%A Jong-Soo Lee
%A Md Bashir Uddin
%A Min-Eun Park
%A Tae-Hwan Kim
%J -
%D 2017
%R 10.1371/journal.ppat.1006398
%X FAS-associated factor-1 (FAF1) is a component of the death-inducing signaling complex involved in Fas-mediated apoptosis. It regulates NF-¦ĘB activity, ubiquitination, and proteasomal degradation. Here, we found that FAF1 positively regulates the type I interferon pathway. FAF1gt/gt mice, which deficient in FAF1, and FAF1 knockdown immune cells were highly susceptible to RNA virus infection and showed low levels of inflammatory cytokines and type I interferon (IFN) production. FAF1 was bound competitively to NLRX1 and positively regulated type I IFN signaling by interfering with the interaction between NLRX1 and MAVS, thereby freeing MAVS to bind RIG-I, which switched on the MAVS-RIG-I-mediated antiviral signaling cascade. These results highlight a critical role of FAF1 in antiviral responses against RNA virus infection
%K Immunoprecipitation
%K Enzyme-linked immunoassays
%K Viral replication
%K RNA viruses
%K Fluorescence microscopy
%K Cytokines
%K Interferons
%K Antiviral immune response
%U https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006398