%0 Journal Article
%T Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus
%A Anja Schneider
%A Christoph Stein
%A Franziska Kleinert
%A Friedrich G£¿tz
%A Gabriele Bierbaum
%A Isabelle Bekeredjian-Ding
%A Julia Uebele
%A Minh-Thu Nguyen
%A Olga Tich¨¢
%J -
%D 2017
%R 10.1371/journal.ppat.1006387
%X Intracellular persistence of Staphylococcus aureus favors bacterial spread and chronic infections. Here, we provide evidence for the existence of human CD4+ and CD8+ T cell memory against staphylococcal antigens. Notably, the latter could provide a missing link in our understanding of immune control of intracellular S. aureus. The analyses showed that pulsing of monocyte-derived dendritic cells (MoDC) with native staphylococcal protein antigens induced release of Th2-associated cytokines and mediators linked to T regulatory cell development (G-CSF, IL-2 and IL-10) from both CD4+ and CD8+ T cells, thus revealing a state of tolerance predominantly arising from preformed memory T cells. Furthermore, G-CSF was identified as a suppressor of CD8+ T cell-derived IFN¦Ã secretion, thus confirming a tolerogenic role of this cytokine in the regulation of T cell responses to S. aureus. Nevertheless, delivery of in vitro transcribed mRNA-encoded staphylococcal antigens triggered Th1-biased responses, e.g. IFN¦Ã and TNF release from both na£¿ve and memory T cells. Collectively, our data highlight the potential of mRNA-adjuvanted antigen presentation to enable inflammatory responses, thus overriding the existing Th2/Treg-biased memory T cell response to native S. aureus antigens
%K T cells
%K Cytotoxic T cells
%K Cytokines
%K Staphylococcus aureus
%K Messenger RNA
%K Memory T cells
%K Enzyme-linked immunoassays
%K Staphylococcus
%U https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006387