%0 Journal Article
%T microRNA dependent and independent deregulation of long non-coding RNAs by an oncogenic herpesvirus
%A Irina Haecker
%A Lauren Appleby Gay
%A Rolf Renne
%A Sunantha Sethuraman
%A Vaibhav Jain
%J -
%D 2017
%R 10.1371/journal.ppat.1006508
%X Kaposi¡¯s sarcoma (KS) is a highly prevalent cancer in AIDS patients, especially in sub-Saharan Africa. Kaposi¡¯s sarcoma-associated herpesvirus (KSHV) is the etiological agent of KS and other cancers like Primary Effusion Lymphoma (PEL). In KS and PEL, all tumors harbor latent KSHV episomes and express latency-associated viral proteins and microRNAs (miRNAs). The exact molecular mechanisms by which latent KSHV drives tumorigenesis are not completely understood. Recent developments have highlighted the importance of aberrant long non-coding RNA (lncRNA) expression in cancer. Deregulation of lncRNAs by miRNAs is a newly described phenomenon. We hypothesized that KSHV-encoded miRNAs deregulate human lncRNAs to drive tumorigenesis. We performed lncRNA expression profiling of endothelial cells infected with wt and miRNA-deleted KSHV and identified 126 lncRNAs as putative viral miRNA targets. Here we show that KSHV deregulates host lncRNAs in both a miRNA-dependent fashion by direct interaction and in a miRNA-independent fashion through latency-associated proteins. Several lncRNAs that were previously implicated in cancer, including MEG3, ANRIL and UCA1, are deregulated by KSHV. Our results also demonstrate that KSHV-mediated UCA1 deregulation contributes to increased proliferation and migration of endothelial cells
%K Long non-coding RNAs
%K MicroRNAs
%K Kaposi's sarcoma-associated herpesvirus
%K Endothelial cells
%K Viral persistence and latency
%K Carcinogenesis
%K Gene expression
%K Microarrays
%U https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006508