%0 Journal Article
%T Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys
%A Amanda D. Melin
%A Babak Shaban
%A Ben Roediger
%A Christopher J. Jolly
%A John E. Pimanda
%A Joseph D. Orkin
%A Justin J.-L. Wong
%A Marcilio Jorge Fumagalli
%A Marcus J. Crim
%A Matthew B. O'Rourke
%A Matthew P. Padula
%A Natalia Pinello
%A Ori Brenner
%A Quintin Lee
%A Renhua Song
%A Simon H. Williams
%A S¨¦bastien Monette
%A William Marciel de Souza
%A Wolfgang Weninger
%J -
%D 2020
%R 10.1371/journal.ppat.1008262
%X Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify ¡°p10¡± and ¡°p15¡± as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that many mammal chapparvoviruses are likely to be nephrotropic
%K Kidneys
%K Polymerase chain reaction
%K Mammalian genomics
%K Gene prediction
%K Bird genomics
%K Parvoviruses
%K Polyadenylation
%K Polypeptides
%U https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008262