%0 Journal Article
%T CD8+ T Lymphocyte Expansion, Proliferation and Activation in Dengue Fever
%A Andr¨¦ia Manso de Matos
%A Claudio Sergio Pannuti
%A C¨¦lia Luiza de Lima Rodrigues
%A Daniela Santoro Rosa
%A Esper George Kallas
%A Evaldo Stanislau Affonso Ara¨²jo
%A Expedito Jos¨¦ Albuquerque Luna
%A Jos¨¦ Eduardo Levi
%A Karina Inacio Carvalho
%A Lucy Santos Villas-Boas
%A Ol¨ªmpia Massae Nakasone Peel Furtado Oliveira
%A Wanessa Cardoso da Silva
%J -
%D 2015
%R 10.1371/journal.pntd.0003520
%X Dengue fever induces a robust immune response, including massive T cell activation. The level of T cell activation may, however, be associated with more severe disease. In this study, we explored the level of CD8+ T lymphocyte activation in the first six days after onset of symptoms during a DENV2 outbreak in early 2010 on the coast of S£¿o Paulo State, Brazil. Using flow cytometry we detected a progressive increase in the percentage of CD8+ T cells in 74 dengue fever cases. Peripheral blood mononuclear cells from 30 cases were thawed and evaluated using expanded phenotyping. The expansion of the CD8+ T cells was coupled with increased Ki67 expression. Cell activation was observed later in the course of disease, as determined by the expression of the activation markers CD38 and HLA-DR. This increased CD8+ T lymphocyte activation was observed in all memory subsets, but was more pronounced in the effector memory subset, as defined by higher CD38 expression. Our results show that most CD8+ T cell subsets are expanded during DENV2 infection and that the effector memory subset is the predominantly affected sub population
%K T cells
%K Cytotoxic T cells
%K Dengue fever
%K Viral load
%K Dengue virus
%K Cell staining
%K Cloning
%K Brazil
%U https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0003520