%0 Journal Article
%T The Coding and Noncoding Architecture of the Caulobacter crescentus Genome
%A Bo Zhou
%A Brandon Williams
%A Gene-Wei Li
%A Harley H. McAdams
%A Jared M. Schrader
%A Jonathan S. Weissman
%A Keren Lasker
%A Lucy Shapiro
%A Sean Crosson
%A Tao Long
%A W. Seth Childers
%J -
%D 2014
%R 10.1371/journal.pgen.1004463
%X Caulobacter crescentus undergoes an asymmetric cell division controlled by a genetic circuit that cycles in space and time. We provide a universal strategy for defining the coding potential of bacterial genomes by applying ribosome profiling, RNA-seq, global 5กไ-RACE, and liquid chromatography coupled with tandem mass spectrometry (LC-MS) data to the 4-megabase C. crescentus genome. We mapped transcript units at single base-pair resolution using RNA-seq together with global 5กไ-RACE. Additionally, using ribosome profiling and LC-MS, we mapped translation start sites and coding regions with near complete coverage. We found most start codons lacked corresponding Shine-Dalgarno sites although ribosomes were observed to pause at internal Shine-Dalgarno sites within the coding DNA sequence (CDS). These data suggest a more prevalent use of the Shine-Dalgarno sequence for ribosome pausing rather than translation initiation in C. crescentus. Overall 19% of the transcribed and translated genomic elements were newly identified or significantly improved by this approach, providing a valuable genomic resource to elucidate the complete C. crescentus genetic circuitry that controls asymmetric cell division
%K Ribosomes
%K Caulobacter crescentus
%K Messenger RNA
%K Operons
%K Protein translation
%K Translation initiation
%K Cell cycle and cell division
%K Sequence motif analysis
%U https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004463