%0 Journal Article
%T Genetic Interaction Mapping Reveals a Role for the SWI/SNF Nucleosome Remodeler in Spliceosome Activation in Fission Yeast
%A Assen Roguev
%A Christine Guthrie
%A Colm J. Ryan
%A Jesse J. Lipp
%A Jiewei Xu
%A Kelly E. Nissen
%A Kristin L. Patrick
%A Michael Shales
%A Nevan J. Krogan
%J -
%D 2015
%R 10.1371/journal.pgen.1005074
%X Although numerous regulatory connections between pre-mRNA splicing and chromatin have been demonstrated, the precise mechanisms by which chromatin factors influence spliceosome assembly and/or catalysis remain unclear. To probe the genetic network of pre-mRNA splicing in the fission yeast Schizosaccharomyces pombe, we constructed an epistatic mini-array profile (E-MAP) and discovered many new connections between chromatin and splicing. Notably, the nucleosome remodeler SWI/SNF had strong genetic interactions with components of the U2 snRNP SF3 complex. Overexpression of SF3 components in ¦¤SWI/SNF cells led to inefficient splicing of many fission yeast introns, predominantly those with non-consensus splice sites. Deletion of SWI/SNF decreased recruitment of the splicing ATPase Prp2, suggesting that SWI/SNF promotes co-transcriptional spliceosome assembly prior to first step catalysis. Importantly, defects in SWI/SNF as well as SF3 overexpression each altered nucleosome occupancy along intron-containing genes, illustrating that the chromatin landscape both affects¡ªand is affected by¡ªco-transcriptional splicing
%K Nucleosomes
%K Introns
%K Schizosaccharomyces pombe
%K Chromatin
%K Genetic interactions
%K Spliceosomes
%K Hyperexpression techniques
%K RNA splicing
%U https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005074