%0 Journal Article
%T The Global Regulatory Architecture of Transcription during the Caulobacter Cell Cycle
%A Bo Zhou
%A Cong B. Dinh
%A David L. Dill
%A Eduardo Abeliuk
%A Harley H. McAdams
%A James Q. Pham
%A Jared M. Schrader
%A Lucy Shapiro
%A Virginia S. Kalogeraki
%A Zhongying Z. Cui
%J -
%D 2015
%R 10.1371/journal.pgen.1004831
%X Each Caulobacter cell cycle involves differentiation and an asymmetric cell division driven by a cyclical regulatory circuit comprised of four transcription factors (TFs) and a DNA methyltransferase. Using a modified global 5กไ RACE protocol, we globally mapped transcription start sites (TSSs) at base-pair resolution, measured their transcription levels at multiple times in the cell cycle, and identified their transcription factor binding sites. Out of 2726 TSSs, 586 were shown to be cell cycle-regulated and we identified 529 binding sites for the cell cycle master regulators. Twenty-three percent of the cell cycle-regulated promoters were found to be under the combinatorial control of two or more of the global regulators. Previously unknown features of the core cell cycle circuit were identified, including 107 antisense TSSs which exhibit cell cycle-control, and 241 genes with multiple TSSs whose transcription levels often exhibited different cell cycle timing. Cumulatively, this study uncovered novel new layers of transcriptional regulation mediating the bacterial cell cycle
%K Cell cycle and cell division
%K Cell binding
%K Gene regulation
%K Sequence motif analysis
%K Caulobacter
%K Transcriptional control
%K Regulator genes
%K Transcription factors
%U https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004831