%0 Journal Article
%T Effect of rosiglitazone on the expression of tumor necrosis factor-¦Á in the liver tissue of mice with non-alcoholic steatohepatitis
%A Lei Wang
%A Xiaodong Yuan
%A Yuemin Nan
%J -
%D 2017
%X The aim of this study was to investigate the effect of rosiglitazone on the expression of Tumor Necrosis Factor-¦Á (TNF-¦Á) in the liver tissue of mice with Non-Alcoholic Steatohepatitis (NASH) and its role in NASH progression. Fifteen healthy male C57BL/6J mice were randomly divided into three groups: the control group (MCD+), the model group (MCD-), and the rosiglitazone group (MCD ¡À R). The serum alanine aminotransferase (ALT) and triglyceride (TG) levels were detected using the enzyme method. Hematoxylin-Eosin (HE) staining was performed to observe steatosis, inflammation, and fibrosis in the liver tissue. Reverse Transcription Polymerase Chain Reaction (RT-PCR) and western blotting were performed to detect the expression of TNF-¦Á mRNA and protein. Liver pathology: the MCD+ group showed no significant liver steatosis, inflammation, and fibrosis; the MCD- group showed obvious steatosis and inflammation. However, the MCD ¡À R group exhibited reduced lesions. The expression of TNF-¦Á mRNA and protein in the liver tissue among the groups was as follows: group MCD ¡Ý group MCD ¡À R>group MCD+. In the mouse models of NASH, the TNF-¦Á expression in the liver tissue was related to the progression of NASH; Rosiglitazone can inhibit the expression of TNF-¦Á and prevent the progression of NASH, thus providing new ideas for selecting therapeutic targets for NASH
%K Non-alcoholic steatohepatitis
%K Tumor necrosis factor-£¿£¿£¿¡À
%K Peroxisome proliferator-activated receptor £¿£¿£¿3 agonist
%K Rosiglitazone
%U https://www.alliedacademies.org/articles/effect-of-rosiglitazone-on-the-expression-of-tumor-necrosis-factoralpha-in-the-liver-tissue-of-mice-with-nonalcoholic-steatohepati-7811.html