%0 Journal Article
%T Histamine induced apoptosis in primary-cultured glioma
%A Aigang Sun
%A Bing Li
%A Chang Fei
%A Feng Guo
%A Jian Zhang
%A Linchun Huan
%A Mei Shao
%A Shouzhong Guo
%A Yuhai Liu
%A Yuquan Jiang
%J -
%D 2017
%X The study aim was to observe the apoptosis induction effects of histamine towards the Primary Cultured Human Glioma Cells (PCHGCs), and to discuss the relationships between the histamine-induced PCHGCs apoptosis and Ca2+ influx. The fresh glioma tissues obtained from the surgery were added with 10% foetal calf serum (FBS) containing 1640 nutrient solution for the cultivation. The cells in good conditions were added into 0.01 mg/ml, 0.5 mg/ml and 0.1 mg/ml histamine media for the primary cell cultivation. The Confocal Laser Scanning Microscope (CLSM) was used to detect the intracellular free Ca2+ concentration, and the Flow Cytometry (FCM) was used to detect the cell apoptosis rate under the effects of different histamine concentrations. PCHGCs that were cultured with 0.5 mg/ml and 0.1 mg/ml histamine solutions had the significant increase of intracellular free Ca2+ concentrations, which induced the apoptosis. The apoptosis occurrence was related to the histamine amount and the cultivation period in a certain extent. Histamine induced the PCHGCs apoptosis by increasing the concentration of intracellular free Ca2+
%K Histamine
%K Primary culture
%K Glioma
%K Apoptosis
%U https://www.alliedacademies.org/articles/histamine-induced-apoptosis-in-primarycultured-glioma-8100.html