%0 Journal Article
%T MicroRNA-320 inhibits insulin resistance in patients with PCOS through regulating ERK1/2 signaling pathway
%A Li Tan
%A Wen-Na Yuan
%J -
%D 2017
%X Objective: This study aimed to investigate the role of microRNA-320 in Insulin Resistance (IR) of patients with Polycystic Ovary Syndrome (PCOS) and its regulatory mechanism.
Methods: Ovarian tissues in 20 patients with PCOS and IR (PCOS-IR group) and 20 normal persons (control group) were collected. The total RNA was extracted from each ovarian tissue. The differential microRNA-320 expression in patients of PCOS-IR group and control group were verified by RT-PCR. TargetScan and miRanda were used to predict the possible specific binding target sites with microRNA-320. The dual-luciferase reporter gene method was used to predict the specific binding target sites of 3¡¯ UTR. The regulatory pathways of ovarian tissues in patients of PCOS-IR group and control group were predicted by the Western blot method.
Results: The microRNA-320 expression in the ovarian tissue in the PCOS¨CIR group was significantly lower than that of the control group. The IRS-1 specific binding target sites with microRNA-320 were screened by the bioinformatic software and dual-luciferase reporter gene method. Moreover, compared with the control group, the expression of ERK1/2 was significantly up-regulated in PCOS group.
Conclusion: MicroRNA-320 could inhibit IR in patients with PCOS through IRS-1 regulating ERK1/2 signaling pathway
%K Microrna-320
%K PCOS
%K Insulin resistance
%K ERK1/2
%U https://www.alliedacademies.org/articles/microrna320-inhibits-insulin-resistance-in-patients-with-pcos-through-regulating-erk12-signaling-pathway.html