%0 Journal Article
%T Effects of DMSO (Dimethyl sulfoxide) free cryopreservation with program freezing using a magnetic field on periodontal ligament cells and dental pulp tissues.
%A H. Koseki
%A H. Sumi
%A J. Ohtani
%A K. Tanne
%A M. Kaku
%A M. Motokawa
%A N. Ohwada
%A S. Abedini
%A S. Kojima
%A T. Fujita
%A T. Kawata
%J -
%D 2012
%X The purpose of this study was to investigate the effects of short-term cryopreservation on the root of an incomplete human tooth with periodontal ligament cells (PDL) and dental pulp tissues. Extracted teeth were cryopreserved for three months using a programmed freezer combined with a magnetic field, known as Cells Alive System ¡°CAS¡±. PDL regeneration and appropriate apexogenesis after transplanting a magnetically cryopreserved immature tooth were clinically confirmed. These findings demonstrate that teeth banking with the use of a magnetic field programmed freezer can be available for future autotransplantation as a treatment modality for replacing missing teeth. PDL regeneration and appropriate apexogenesis after transplanting a magnetically cryopreserved immature tooth were clinically confirmed. These findings demonstrate that teeth banking with the use of a magnetic field programmed freezer can be available for future autotransplantation as a treatment modality for replacing missing teeth. Results indicate that short-term cryopreservation with the use of a CAS freezer does not affect the rate of recovery or characteristics of PDL cells
%K Cryopreservation
%K root of an incomplete human tooth
%K autotransplantation
%K PDL cell
%K pulp cell
%K magnetic
field
%U https://www.alliedacademies.org/articles/effects-of-dmso-dimethyl-sulfoxide-free-cryopreservation-with-program-freezing-using-a-magnetic-field-on-periodontal-ligament-cell.html