%0 Journal Article
%T The effect and its mechanism of Morinda officinalis how polysaccharide on chondrocyte apoptosis induced by IL-1¦Â
%A Huang Yong-hong
%A Zhang Jian-lin
%A Zhang Ru-guo
%J -
%D 2017
%X Objective: To evaluate the effect and its mechanism of Morinda officinalis how polysaccharide on chondrocyte catabolism and apoptosis induced by IL (Interleukin)-1¦Â.
Materials and Methods: Chondrocytes were randomly divided into 5 groups, control group, IL-1 beta group (50 ¦Ìg/ml IL-1¦Â in chondrocyte for 24 h), Morinda officinalis how polysaccharide group (2 ¦Ìg/ml, 5 ¦Ìg/ml and 10 ¦Ìg/ml Morinda officinalis how polysaccharide adding to the chondrocyte). The apoptosis rate of the five groups was calculated and the relative expression of Bcl-2 (B Cell Leukemia 2), Bax (Bcl-2 Associated X Protein) and Caspase 9 were quantity by PCR and Western-blot. Elisa kit was used to measure the expression of iNOS in each group.
Results: 50 ¦Ìg/ml IL-1¦Â can significantly increasing the iNOS and apoptosis rates compared to the control group, and the difference was statistically significant (p<0.05), 10 ¦Ìg/ml Morinda officinalis how polysaccharide can significantly reduce the chondrocytes apoptosis and iNOS level, the difference was statistically significant (p<0.05). The gene expression and protein expression of Bax and Caspase 9 in IL-1¦Â group was higher than control group, and the Bcl-2 is lower than control group, the differences were statistically significant (p<0.05). Morinda officinalis how polysaccharide in 10 ¦Ìg/ml can significantly reduce the gene expression and protein expression of Bax and Caspase 9 and down the expression of Bcl-2, the differences were statistically significant (p<0.05).
Conclusion: Morinda officinalis how polysaccharide can increase the expression of Bcl-2, reduce the expression of Bax and Caspase 9, thus can prevent chondrocytes apoptosis induced by IL-1¦Â
%K Morinda officinalis how polysaccharide
%K Chondrocyte
%K Apoptosis
%U https://www.alliedacademies.org/articles/the-effect-and-its-mechanism-of-morinda-officinalis-how-polysaccharide-on-chondrocyte-apoptosis-induced-by-il1beta-7836.html