%0 Journal Article
%T Qiliqiangxin improves oxidized low-density lipoprotein-induced human coronary artery endothelial cells injury
%A Changjiang Du
%A Guangyuan Meng
%A Haijun Cao
%A Haiqin Lv
%A Xiujuan Wang
%J -
%D 2018
%R 10.4066/biomedicalresearch.29-17-3435
%X Qiliqiangxin (QL) has protective effect for the cardiovascular diseases treatment. This study aim to evaluate the effect of QL on the cell viability, cell apoptosis and caspase-3 activity in oxidized LDL (oxLDL)-induced Human Coronary Artery Endothelial Cells (HCAECs). HCAECs were treated with QL (0-1¦Ìg/ml) and oxLDL (150 ¦Ìg/ml). The HCAECs viability was detected by cell proliferation assay and Lactate Dehydrogenase (LDH) release assay. The HCAECs apoptosis was evaluated by the annexin V-FITC assay. The activity of caspase-3 of HCAECs was measured by colorimetric caspase-3 assay. Cell migration assay and capillary-like tube formation assay on Matrigel were performed. Treatment of HCAECs to ox-LDL (150 ¦Ìg/ml) decreased cell viability, stimulated apoptosis and enhanced caspase-3 activity. In addition, treatment with QL (0.5 ¦Ìg/ml) alone did not affect cell viability and LDH release. Furthermore, the treatment with QL (0.5 ¦Ìg/ml) significantly increased ox-LDL (150 ¦Ìg/ml) decreased cell viability. Treatment with QL (0.5 ¦Ìg/ml) reduced ox-LDL-stimulated apoptosis and caspase-3 activity in HCAECs in a dose-dependent manner. QL (0.5 ¦Ìg/ml) attenuated ox-LDL (150 ¦Ìg/ml) abolished cell migration and tube formation. Our study demonstrated that QL prevents ox-LDLinduced HCAECs injury by decreasing the apoptosis via caspase-3 activity
%K Qiliqiangxin
%K Oxidized LDL
%K Apoptosis
%K Human microvascular endothelial cells
%K Caspase-3
%U https://www.alliedacademies.org/articles/qiliqiangxin-improves-oxidized-lowdensity-lipoproteininduced-human-coronary-artery-endothelial-cells-injury-9781.html