%0 Journal Article
%T Nicotinic acid, lauric acid and kaempferol abolish ATP-binding cassette
transporter subfamily A member 1 (ABCA1) down-regulation by TNF-?
in hepatocarcinoma HepG2 cell line.
%A Choy-Hoong Chew
%A Hoay-Yan Cheah
%A Hong-Kin Wong
%A Wyi-Sian Lim
%A Yew-Yen Wong
%J -
%D 2014
%X The aim of this study was to investigate the effect of different concentrations of antioxidants on ABCA1 mRNA expression in TNF-¦Á stimulated hepatocarcinoma (HepG2) cell line. TNF- ¦Á-stimulated cells were treated with 1 ¦ÌM, 5 ¦ÌM, 10 ¦ÌM and 20 ¦ÌM of nicotinic acid, lauric acid or kaempferol for 24 h, respectively. Real time RT-PCR was carried out to analyze the expression of the genes. Relative ABCA1 mRNA levels were normalized to beta-actin and their relative expression to untreated cells was analyzed using delta-delta CT method. Here we show that ABCA1 mRNA levels were significantly suppressed by 20 ng/ml of TNF-¦Á, but all three antioxidants were able to antagonize this suppressive effect of TNF-¦Á. High doses (20 ¦ÌM) of the antioxidants significantly up-regulated the ABCA1 gene expression. This study successfully profiled that all the three antioxidants were able to alleviate the downregulatory effect of TNF-¦Á on ABCA1 transcript level in HepG2 cells. Although stimulation with kaempferol alone induced ABCA1 expression to high levels, its ability to reduce TNF-¦Á inhibitory response was the weakest amongst the three antioxidants
%K Nicotinic acid
%K lauric acid
%K kaempferol
%K ABCA1
%K TNF-£¿¡À
%K expression
%U https://www.alliedacademies.org/articles/nicotinic-acid-lauric-acid-and-kaempferol-abolish-atpbinding-cassettetransporter-subfamily-a-member-1-abca1-downregulation-by-tnfi.html