%0 Journal Article
%T Examining the relationship between MCPH1 with H2AX in esophageal cancer cells ECA109
%A Hongyun Shi
%A Ke Zhang
%A Ming Wen
%A Yu Yuan
%J -
%D 2017
%X Objective: To discover the role of MCPH1 in the DNA double-strand damage induced by ionizing radiation and the relationship with H2AX in esophageal cancer cell ECA109.
Methods: ECA109 cancer cells will be accepted 8 Gy 1 h after irradiation for protein extraction and immunofluorescence observed MCPH1 and H2AX protein expression and nuclear foci changes. Establish a stable low expression of H2AX cell lines. Detecting MCPH1 and H2AX protein expression and nuclear foci changes induced by ionizing radiation after silence H2AX.
Results: 1. Successfully constructed the ECA109 cell line with silence H2AX gene. 2. Ionizing radiation could cause r-H2AX and MCPH1 protein expression increase, as the same as nuclear focus increase of r- H2AX and MCPH1. 3. The protein level and nucleus focus of r-H2AX and MCPH1 are significantly reduced in ECA109 after silence H2AX.
Conclusion: MCPH1 is the part of DNA damage response triggered by ionizing radiation and is located in the damage response downstream and can be regulated by H2AX
%K Esophageal cancer
%K Ionizing radiation
%K DNA double-strand breaks
%K £¿£¿£¿£¿£¿£¿£¿3-H2AX
%K MCPH1
%U https://www.alliedacademies.org/articles/examining-the-relationship-between-mcph1-with-h2ax-in-esophageal-cancer-cells-eca109-8960.html