%0 Journal Article
%T Production, Optimization and Characterization of £¿¡À-Galactosidase by <i>Bacillus</i> sp. SPE10 and <i>Bacillus</i> sp. SPE15 Isolated from Soy Effluent
%A Andal Priyadharshini D
%A Damodaran R
%A Mukesh Kumar DJ
%A Saranya GM
%A Suresh K
%J -
%D 2012
%X This study evaluated ¦Á-galactosidase production by two Bacillus strains SPE10 and SPE15 isolated from effluent sample. Various physico-chemical parameters such as pH, temperature, incubation period, and effect of different carbon and nitrogen sources were studied in order to determine the optimum conditions for ¦Á-galactosidase production by both the strains. The incubation time of 72 h and 50¡ãC with the presence of raffinose and peptone favoured ¦Á-galactosidase production in both the strains. pH 8 and 7 were found to be optimum for SPE10 and SPE15 respectively. Purified enzymes showed molecular weights of 81 and 78 kDa revealed by SDS-PAGE
%K Mukesh Kumar DJ
%K Suresh K
%K Saranya GM
%K Andal Priyadharshini D
%K Damodaran R and Kalaichelvan PT
%K Alpha galactosidase
%K Bacillus
%K raffinose
%K peptone
%K SDS-PAGE
%U https://www.imedpub.com/abstract/production-optimization-and-characterization-of-galactosidase-by-ibacillusi-sp-spe10-and-ibacillusi-sp-spe15-isolated-from-soy-effluent-16397.html