%0 Journal Article
%T Serological Proteome Analysis for Identification of Potential Antigen in Atopic Dermatitis
%A Blendi Ura
%A Federica Scrimin
%A Giorgio Arrigoni
%A Irene Berti
%A Luigina De Leo
%A Tarcisio Not
%J -
%D 2016
%R 10.21767/2574-2817.100004
%X Objective: Identify human skin derived auto antigens that   could be recognized by IgE serum of patients with both   severe atopic dermatitis and high serum IgE   immunoglobulins.
Design: The study was performed retrospectively on sera   collected from children with severe atopic dermatitis with   total IgE serum concentration (IgE>3000 U.I./ml), as a   serum sample controls we used the children¡¯s sera   suffering from severe food allergy and with high IgE   concentrations against food proteins (IgE>90 kU/l). All   these serum samples were used to identify skin-derived   antigen by using immunoproteome technique.
Setting: Institute for Maternal and Child Health.
Patients: Six children suffering from severe atopic   dermatitis and six suffering from severe food allergy   without skin involvement.
Interventions: Serological proteome analysis (SERPA) for   antigen identification.
Main outcome measures: First immunoproteomic study   for identification of potential antigen in severe atopic   dermatitis. Identification of two immunoreactive proteins   IGKC (Ig kappa chain C region) and IGLC1 (Ig lambda-1   chain C regions).
Results: After membranes analysis we identified two   immunoreactive proteins IGKC and IGLC1 present only in membrane treated with severe atopic dermatitis.   Reaction of these proteins were considered non-specific   event and didn¡¯t further investigate.
Conclusions: Using SERPA analysis we identified two   immuno-reactive proteins. Even these proteins have   proved to be non-specific event; this showed the great   potential of SERPA for antigen identification.
%K Atopic dermatitis
%K Antigen
%K Serological proteome analysis
%K list of open access journals
%K open access
%K open access journals
%K open access publication
%K open access publisher
%K open access publishing
%K open access journal articles
%K imedpub
%K imedpub publishing
%K insight medical publishing
%K imedpub online
%U https://www.imedpub.com/articles/serological-proteome-analysis-for-identification-of-potential-antigen-in-atopic-dermatitis.php?aid=17837