%0 Journal Article
%T A comparative study on cytotoxicity and apoptotic activity of pyocyanin
produced by wild type and mutant strains of Pseudomonas aeruginosa
%A Adawia Al-Saadi
%A Hayfa H Hassani
%A Husam M Hasan
%A Maeda H
Muhammad
%J -
%D 2012
%X Nutrient agar medium with various concentrations of cefotaxime was used for isolation spontaneous mutants from wild type strain of P.aeruginosa PHA-1. Eighty-two mutants were successfully isolated with the viable count 52กม107, these mutants were confirmed as spontaneous not physiological adaption mutants by reculture on the same medium. Then, wild type PHA-1 and mutants were examined for production pyocyanin; a blue greenish pigment was clearly noticed on King A medium. Remarkably the mutant strain named S300-8 was distinguished in productivity in comparison with wild type strain PHA-1; the amount of pigment was 56.0667mg/l and 74.53mg/l respectively. In addition, pyocyanin produced by mutant strain S300-8 revealed a potent efficacy against growth of cancer cell line RD; the low concentration of this pigment caused 65% of dead cells after 72hrs of incubation whereas the cytotoxicity was improved by increasing the concentration of pigment with period of exposure time. Moreover, pyocyanin from mutant strain S300-8 induced apoptosis more than wild type PHA-1; the percentage of DNA fragmentation in RD cell was 80% and 73.3% respectively
%K Hayfa H Hassani
%K Husam M Hasan
%K Adawia Al-Saadi
%K Amal M Ali
%K Maeda H
Muhammad
%K P.aeruginosa
%K Pyocyanin
%K Antiproliferation
%K Apoptosis
%K Spontaneous Mutants
%U https://www.imedpub.com/abstract/a-comparative-study-on-cytotoxicity-and-apoptotic-activity-of-pyocyaninrnproduced-by-wild-type-and-mutant-strains-of-pseudomonas-aeruginosa-15359.html