%0 Journal Article
%T Estimation and Validation of A Polypill
Containing Amlodipine Besylate,
Hydrochlorothiazide and Telmisartan by RPHPLC
%A Rajendra P Rathnam
%J -
%D 2015
%X Objective: A new, simple, accurate RP-HPLC method was developed for the simultaneous estimation of drugs in a polypill containing amlodipinebesylate, hydrochlorothiazide and telmisartan by RP-HPLC. Method: Good chromatographic separation was accomplished in isocratic mode using mixed phosphate buffer: acetonitrile (55:45) as mobile phase, and Kromasil (250 mm ¡Á 4.6 mm i.d, 5 ¦Ìm particle size) column as stationary phase havinga flow rate of 1.0 mL/min at detection wavelength of 254 nm. Results: The time of retention for hydrochlorothiazide, amlodipine and telmisartan was established at 3.2 min, 4.5 min and 5.7 min respectively. This method was validated as per ICH guidelines.21,22 Conclusion: This low cost method was proved to be successful when applied to pharmaceutical dosage forms without any interference in the chromatogram
%K Rajendra P Rathnam and Selina Sravanthi
%K Polypill
%K RP-HPLC
%K Amlodipine Besylate
%K Hydrochlorothiazide
%K Telmisartan
%U https://www.imedpub.com/abstract/estimation-and-validation-of-a-polypillrncontaining-amlodipine-besylaternhydrochlorothiazide-and-telmisartan-by-rphplc-11054.html