%0 Journal Article
%T Effect of plasmin digest of £¿o-casein on survival of some pathogenic and probiotic bacteria
%A Hamid Ezzatpanah
%A Maryam Tajabadi Ebrahimi
%A Massod Mashhadi Akbar Boojar
%A Mehdi Aminafshar
%J -
%D 2013
%X The aim of this work was to evaluate the presence of antibacterial properties in a plasmin digest of bovine ¨°£¿-casein. Although native bovine ¦Ê-caseinis resistant to plasmin, extensive hydrolysis of this milk protein was observed applying a long incubation period. The antibacterial potential of ¦Ê-casein, plasmin, PD¨°£¿ was evaluated against pathogenic (Escherichia coli and Staphylococcus aureus) and probiotic (Lactobacillus casei and Lactobacillus acidophilus) bacteria in vitro. Although ¨°£¿-casein and plasmin had no antibacterial activity, PD¨°£¿ showed antibacterial property against the tested bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PD¨°£¿ was determined for the target bacteria. The MIC and MBC of PD¨°£¿ against Escherichia coli was considerably higher than Staphylococcus aureus, Lactobacillus casei and Lactobacillus acidophilus bacteria. The growth curves alterations of target bacteria in the presence of PD¨°£¿ were monitored by turbidimetry in broth culture. The effect of PD¨°£¿ on lag time and maximum absorbance was more significant than slope of tested bacteria. In addition of growth curves, PD¨°£¿ has inhibitory effects on the plate count confirmation of tested bacteria. The maximum inhibitory effect of PD¨°£¿ was created in MIC concentration
%K Marjaneh Sedaghati
%K Hamid Ezzatpanah
%K Massod Mashhadi Akbar Boojar
%K Maryam Tajabadi Ebrahimi and Mehdi Aminafshar
%K Bovine
%K K-casein
%K Antibacterial
%K Plasmin
%K Growth curve
%U https://www.imedpub.com/abstract/effect-of-plasmin-digest-of-casein-on-survival-of-some-pathogenic-and-probiotic-bacteria-16165.html