%0 Journal Article
%T A validated RP-HPLC method for the determination of Eplerenone in human plasma
%A B. Jyothirmai
%A B. SyamaSundar
%A Satyadev T. N. V. S. S.
%J -
%D 2015
%X A highly sensitive and accurate HPLC method was developed for the determination of Eplerenone in human plasma using Hydrchlorothiazide as internal standard. Diethyl ether was used as solvent in the liquid ¨C liquid extraction process. A non-polar Phenomenex Prodigy ODS-2, C18 column (150 X 4.6 mm id) was chosen as the stationary phase and a binary mixture of 20 mM Sodium acetate buffer (pH 4.0 ¡À 0.05) and methanol in a ratio of 30:70 v/v was used as mobile phase. The drug and the internal standard were eluted under isocratic condition at a flow rate of 1.0 mL/min of the mobile phase and the wavelength of detection is 220 nm (UV ¨C detector). The injection volume is 20 ¦ÌL and the runtime of the method is 6 minutes. A good linearity was observed for the method over the range of 52.52 to 3089.48 ng/mL. The recovery of Eplerenone was 45.48 % with a standard deviation of 1.62 and recovery of internal standard was 75.32%. The LOD of Eplerenone was 52.52 ng/mL. Matrix effects were not observed
%K B. Jyothirmai
%K B. SyamaSundar
%K Satyadev T. N. V. S. S.and Tata Santosh
%K Eplerenone
%K Anti-hypertensive
%K RP ¨C HPLC
%K Isocratic elution etc.
%U https://www.imedpub.com/abstract/a-validated-rphplc-method-for-the-determination-of-eplerenone-in-human-plasma-11681.html