%0 Journal Article
%T Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Rabrprazole and Domperidone in Pharmaceutical Dosage Form
%A Arunadevi S. Birajdar
%A Subramania N. Meyyanathan
%J -
%D 2010
%X Two simple, accurate and sensitive analytical methods have been developed for the simultaneous determination of Rabeprazole and Domperidone in pharmaceutical solid dosage form. The first method involves UV-spectrophotometric determination of both drugs in combination, using Qanalysis method. It involves absorbance measurement at 291.6 nm (¦Ëmax of Rabeprazole) and 240 nm isobestic point in solvent (0.1N NaoH), Sodium hydroxide. The method obeys Beer¡¯s law over linearity range for Rabeprazole 2 ¨C 12 ¦Ìg mL-1 and for Domperidone 3-18 ¦Ìg mL-1 respectively. The second method involves HPLC separation of Rabeprazole and Domperidone drugs in reverse phase mode using Phenomenx C18 column. Linearity was obtained in the concentration range of 0.5-5.0 ¦Ìg mL-1 for Rabeprazole and 0.75-7.5 ¦Ìg mL-1 Domperidone. The detection wavelength was 220 nm. Pantaprazole was used as internal standard in10 ¦Ìg mL¨C1 concentration. Relative standard deviation (R.S.D) for both methods was found <2.0%.The proposed methods were successively applied to muticomponent formulation containing both the drugs. The methods were validated according to ICH guidelines
%K Arunadevi S. Birajdar
%K Subramania N. Meyyanathan
%K Bojraj Suresh
%K Rabeprazole
%K Domperidone
%K Pantaprazole
%K UV- spectrophotometry
%K RP-HPLC
%U https://www.imedpub.com/abstract/application-of-uvspectrophotometry-and-rphplc-for-simultaneous-determination-of-rabrprazole-and-domperidone-in-pharmaceutical-dosage-form-16932.html