%0 Journal Article
%T Validated RP-HPLC method for simultaneous estimation of metformin hydrochloride and sitagliptin phosphate in bulk drug and pharmaceutical dosage form
%A Deepali Gangrade
%J -
%D 2015
%X A new simple isocratic RP-HPLC method was developed for the simultaneous estimation of Metformin Hydrochloride and Sitagliptin Phosphate in tablet formulation. The separation was achieved on SUPELCO C-18 analytical column (150cm ¡Á4.6mm I.D., 3¦Ìm particle size) in isocratic elution mode with the mobile phase consisting of 10mM Potassium dihydrogen ortho phosphate (pH:3.00): Acetonitrile in the ratio of 65:35 v/v and the column was maintained at 25¡ãC. The detection of eluent from the column was detected using UV detector at 256nm and the flow rate was maintained at 1.0 ml/min. The proposed method has permitted the quantification of Metformin Hydrochloride and Sitagliptin Phosphate in the linearity range of 100-300¦Ìg/ml and 10-30¦Ìg/ml. The method was validated in terms of system suitability, specificity, linearity and precision. The proposed method can be successfully applied in the quality control of bulk and pharmaceutical dosage forms
%K Deepali Gangrade and Amit Sharma
%K Metformin Hydrochloride
%K Sitagliptin Phosphate
%K HPLC
%K Isocratic elution
%K Method validation.
%U https://www.imedpub.com/abstract/validated-rphplc-method-for-simultaneous-estimation-of-metformin-hydrochloride-and-sitagliptin-phosphate-in-bulk-drug-and-pharmaceutical-dosage-form-11614.html