%0 Journal Article
%T Genotypic Detection of Cefepime Resistance in Iraqi Clinical Isolates of Pseudomonas aeruginosa
%A Israa Mohamed Safi Al-Kadmy
%A Sawsan Sajid AL-Jubori
%J -
%D 2014
%X Study background and aims: Pseudomonas aeruginosa is opportunistic pathogen commonly implicated in serious nosocomial infections .It resisted many Antibiotics including ¦Â-lactams group. The aims of this study were to detect ESBLs production in clinically isolated P. aeruginosa and the prevalence of Cefepime resistance gene using PCR technique.  Material and method: Eighteen isolates of P. aeruginosa were isolated from patients suffering various infections. They diagnosed using Api 20E Kit followed by genotypic detection using a housekeeping gene (rpsl) by PCR. Rapid ESBLs detection kit was used to detect four types of ¦Â-lactamases including: preliminary screening for ¦Â-lactemase, ES¦ÂLs type, Metallo-¦Â- lactamases (M¦ÂL) and AmpC enzyme.  BlaCTX-M and blaCMY genes responsible for 3rd and 4th generation cephalosprine (cefepime) resistance respectively were screened by PCR.  Results: Out of 18 isolates,15(83.3%) were positive in Preliminary screening of ¦Â-lactamases, ES¦ÂLs type were detected in 13 isolates (72.2%), the rate of  M¦ÂL producers were 9(50%) and 7 isolates (38.8%) were  AmpC producers. Detection of blaCTX-M gene revealed that 13\18 isolates (72.2%) harbored this gene, while prevalence of blaCMY gene was 3\18 (16.6%). Conclusion: Most of the isolates were able to produce more than one type ¦Â-lactamases   and 3rd generation resistance is more predominant as compared with 4th generation resistance
%K Sawsan Sajid AL-Jubori
%K Israa Mohamed Safi Al-Kadmy and Eman Natiq Naje
%K ESBLs of P. aeruginosa
%K BlaCTX-M gene
%K BlaCMY gene
%U https://www.imedpub.com/abstract/genotypic-detection-of-cefepime-resistance-in-iraqi-clinical-isolates-of-pseudomonas-aeruginosa-10654.html