%0 Journal Article %T <i>In Vitro</i> Conservation Tactics for a Sweet Genotype of <i>Amygdalus scoparia</i>, an Endangered Medicinally Important Tree Species by Propagation Using Synthetic Seeds %A Zahra Alirezaei %A Marzieh Afazel %A Siamak Shirani Bidabadi %J Journal of Biosciences and Medicines %P 208-219 %@ 2327-509X %D 2020 %I Scientific Research Publishing %R 10.4236/jbm.2020.811019 %X
Bud stems arisen from in vitro cultures of A. scoparia were encapsulated in calcium alginate pieces for short term stockpile and germplasm interchange. The maximum frequency (88.96%) of conversion of encapsulated nodal segments into plantlets and the highest node number (7.20) was performed on a murashige and Skoog¡¯s medium (MS medium) containing 2.2 ¦ÌM banzyl adenine (BA). The highest length of re-growing shoots was achieved when MS medium was supplied with 2.2 ¦ÌM BA and 0.5 ¦ÌM NAA. However, the number of shoots produced was higher (5.30 and 5.10) on MS medium supplemented with 2.2 ¦ÌM BA and 0.5 ¦ÌM NAA and MS medium with 2.2 ¦ÌM BA, respectively than on the hormone-free media. Treatment with 19.6 ¦ÌM IBA resulted in the highest conversion of encapsulated nodal segments into plantlets. The frequency of conversion (89.6% - 88.6%) was retained at 25?C for up to 2 weeks without significant change. The highest frequencies (61.1%) of plantlet formation from encapsulated nodal segments were obtained by transferring synthetic seeds onto peat mass and perlite (2:1) (v/v) mixture substrate. When transplanted into the peat mass and perlite (2:1) (v/v) mixture, these plantlets showed greater plantlet high, leaf number, shoot number and root number per plantlet than those of the other substrates. The synthetic seed technology offered a promising way for short term storage without refrigerating, germplasm conservation exchange for improvement and an alternative clonal propagation method for this endangered genotype of A. scoparia.
%K < %K i> %K Amygdalus scoparia< %K /i> %K Micropropagation %K Plantlet Conversion %K Short Term Storage %K Synthetic Seeds %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=104617