%0 Journal Article
%T Expression, Purification and Crystallization of Wheat Profilin (Tri a 12)
%A Cichna-Markl
%A Margit
%A Ecker
%A Christina
%A Forstenlechner
%A Peter
%A Hoffmann-Sommergruber
%A Karin
%A Matkovi£¿-£¿alogovi£¿
%A Dubravka
%A Mili£¿
%A Dalibor
%J -
%D 2011
%R 10.5562/cca1790
%X Sa£¿etak Profilin from wheat (Triticum aestivum) has been identified as an allergen (Tri a 12). The recombinant 14 kDa protein was produced in Escherichia coli, purified and crystallized using the hangingdrop vapour-diffusion method. A diffraction-data set was collected in-house from a single crystal to a resolution of 3.3 £¿. The crystals belonged to space group P3221, with unit-cell parameters a = b = 58.9 £¿, c = 82.5 £¿, ¦Á = ¦Â = 90¡ã and ¦Ã = 120¡ã. (doi: 10.5562/cca1790
%K wheat profilin
%K expression
%K crystallization
%K food allergy
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=111037