%0 Journal Article
%T Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS
%A Bui
%A Annam¨¢ria
%A D£¿rnyei
%A ¨¢gnes
%A Kil¨¢r
%A Anik¨®
%A Kil¨¢r
%A Ferenc
%A Kocsis
%A B¨¦la
%A Kov¨¢cs
%A Krisztina
%A Po¨®r
%A Vikt¨®ria
%J -
%D 2011
%R 10.5562/cca1795
%X Sa£¿etak The carbohydrate composition of the rough-type endotoxins (lipopolysaccharides, LPSs) from Shigella sonnei mutant strains (Shigella sonnei phase II - 4303, 562H, R41 and 4350) was investigated by capillary electrophoresis and GC-MS. The monosaccharides obtained by hydrolysis were determined by capillary electrophoresis combined with laser induced fluorescence detection (CE-LIF) after labeling with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) and by GC-MS as alditol-acetate derivatives. It was obtained that the lipopolysaccharides of the isogenic rough mutants are formed in a step-like manner, containing no heptose (4350), one D-glycero-D-mannoheptose (562H), or two or three L-glycero-Dmannoheptoses (R41, 4303, respectively) in the deep core region. Besides the heptoses, the longest LPS from the mutant Shigella sonnei 4303 contains hexoses, such as glucoses and galactoses, in a proportion of approximately 3:2. This study provides a comprehensive comparison of the variability in the carbohydrate part of the rough endotoxins extracted from Shigella sonnei isogenic mutants. (doi: 10.5562/cca1795
%K lipopolysaccharide structure
%K Shigella sonnei rough mutant
%K D-glycero-D-mannoheptose
%K capillary electrophoresis
%K CE-LIF
%K GC-MS
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=111007