%0 Journal Article
%T Lipophilicity determination of acidic compounds: MEEKC as a reliable high-throughput methodology
%A Red¨®n
%A L¨ªdia
%A Ros¨¦s
%A Mart¨ª
%A Subirats
%A Xavier
%J -
%D 2018
%R 10.5599/admet.491
%X Sa£¿etak In the present study a pressure-assisted MEEKC method with reversed-polarity using a conventional CE instrument with UV detection and uncoated fused silica capillaries is validated as a high-throughput methodology for the lipophilicity determination of the neutral species of acidic compounds (pKa > 3.5). After the calibration of the system with four standard compounds of known log Po/w, mass distribution ratios (log kMEEKC) of new molecules can be directly converted into log Po/w values by means of a simple linear equation (log Po/w=a¡¤log kMEEKC+b). The method was internally and externally validated for a log Po/w range between -1.54 and 4.75, with higher accuracies than conventional liquid chromatographic methods
%K Capillary electrophoresis
%K hydrophobicity
%K log Po/w
%K microemulsion electrokinetic chromatography
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=296739