%0 Journal Article %T Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars %A Bauer %A Nata£¿a %A Jagi£¿ %A Mateja %A Leljak Levani£¿ %A Dunja %A Malenica %A Nenad %A Pavleti£¿ %A Bruno %A Von£¿ina %A Darko %A Zduni£¿ %A Goran %J - %D 2020 %R 10.37427/botcro-2020-008 %X Sa£¿etak Most Croatian indigenous grapevine cultivars and vineyards are infected with a few dominant viruses. The goal of this study was to establish somatic embryogenesis as an efficient method for virus elimination from valuable Croatian cultivars and creating a reliable source of healthy plants. Somatic embryogenesis was induced from immature anthers and somatic embryogenesis-derived plantlets for seven indigenous cultivars were successfully regenerated. This procedure led to the elimination of viruses GFLV, GLRaV-1, GLRaV-3 and GFkV that were initially detected in the field-grown cultivars ¡®Plavac mali¡¯ and ¡®Babica¡¯ with an elimination success of at least 30%. The described method has the potential for production of virus-free rooted plantlets for all economically important cultivars or superior cultivar clones and for the establishment of a steady source of certified virus-free planting material %K Babica %K Croatian grapevine cultivars %K plavac mali %K RT-PCR %K somatic embryogenesi %K virus elimination %K virus-free plants %K Vitis vinifera L. %U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=339981