%0 Journal Article
%T Validated LC-ESI-MS/MS method for the determination of ivosidenib in 10 ¦ÌL mice plasma: application to a pharmacokinetic study
%A Dittakavi
%A Sreekanth
%A Jairam
%A Ravi Kumar
%A Jat
%A Rakesh Kumar
%A Mallurwar
%A Sadanand Rangnathrao
%A Mullangi
%A Ramesh
%J -
%D 2019
%R 10.5599/admet.648
%X Sa£¿etak A simple, selective and rapid LC-ESI-MS/MS method has been developed and validated for the quantification of ivosidenib in mice plasma using warfarin as an internal standard (I.S.) as per regulatory guideline. Sample preparation was accomplished through a simple protein precipitation process. Chromatography of ivosidenib and the I.S. was achieved on an Atlantis dC18 column using an isocratic mobile phase comprising 0.2 % formic acid in water and acetonitrile (25:75, v/v) delivered at a flow rate of 1.0 mL/min. LC-MS/MS was operated under the multiple reaction-monitoring mode (MRM) using the electrospray ionization technique in positive ion mode and the transitions of m/z 583.1¡ú186.1 and m/z 309.2¡ú251.3 were used to quantitate ivosidenib and the I.S, respectively. The total chromatographic run time was 2.0 min. Linearity was established in the concentration range of 1.10-3293 ng/mL (r2>0.99). The intra- and inter-day accuracy and precision for ivosidenib in mice plasma were in the range of 5.72-9.91 and 5.90-10.7 %, respectively. Ivosidenib was found to be stable on bench-top for 6 h, up to three freeze-thaw cycles, in in-injector for 24 h and for one month at -80 ¡ãC. The applicability of the validated method has been demonstrated in a mice pharmacokinetic study. Following intravenous (2 mg/kg) and oral (5 mg/kg) administration of ivosidenib to mice, concentrations were quantifiable up to 24 and 48 h, respectively. The bioavailability was 61 %
%K Ivosidenib
%K LC-MS/MS
%K method validation
%K mice plasma
%K pharmacokinetics
%K bioavailability
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=319233