%0 Journal Article
%T Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection
%A Achim
%A Marcela
%A Imre
%A Silvia
%A Muntean
%A Dana
%A Muntean
%A Daniela-Lucia
%A Vlase
%A Laurian
%J -
%D 2011
%R 10.5562/cca1761
%X Sa£¿etak A new simple, sensitive and LC-MS/MS method for quantification of spironolactone and its metabolite, canrenone, in human plasma is proposed. The analytes were analysed on a C18 column at 48 oC, by using a mobile phase of 58 % methanol, 42 % 10 mmol dm£¿3 ammonim acetate in water and a flow rate of 1 mL/min. The detection of both analytes in plasma was performed as follows: ESI+, EIC (m/z 169;187;283;305) from m/z 341, after protein precipitation with methanol. Calibration curves were generated over the ranges 2.77¨C184.50 ng/mL for spironolactone and 2.69¨C179.20 ng/mL for canrenone by using a weighted (1/y) linear regression. The absolute values of within- and between-run precision and accuracy for both analytes ranged between 3.1 and 13.9 %, and the mean recovery was 99.7 %. The analytes demonstrated good stability in various conditions. The validated method has been applied to a bioequivalence study of 50 mg spironolactone tablets on healthy volunteers. (doi: 10.5562/cca1761
%K spironolactone
%K canrenone
%K LC-MS/MS
%K pharmacokinetics
%K plasma
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=111002