%0 Journal Article
%T Phenotype analysis of CD3+CD16+ lymphocytes in the peripheral blood of pigs
%A Andriˋiˋ
%A Miroslav
%A Bendelja
%A Kreˋo
%A Boˋiˋ
%A Frane
%A Cvetiˋ
%A ˋeljko
%A Perak Junakoviˋ
%A Eleonora
%A Terziˋ
%A Svjetlana
%A Valpotiˋ
%A Hrvoje
%A Vujnoviˋ
%A Anja
%A ˋandor
%A Ksenija
%A ˋarkoviˋ
%A Irena
%J -
%D 2020
%R 10.46419/vs.51.2.2
%X Saˋetak The phenotype of porcine peripheral blood T cells and natural killer (NK) cells has been well-studied over the past three decades, though porcine peripheral blood lymphocytes with mixed T/NK-cell phenotype within perforin- and NKp46- positive CD3+ populations have also been identified. Despite the mixed phenotype, both populations showed in vitro NK cell-like major histocompatibility complex-unrestricted cytolysis. In this study, the peripheral blood lymphocytes of 15 crossbreed, 12-week-old pigs of both sexes, were analysed by flow cytometry for the expression of leukocyte surface antigens (cluster of differentiation, CD) that can be found on porcine T cells (CD3, TCR-污汛 and CD4), NK cells (CD16) or on both cell populations (CD8汐 and SLA-DR). We found the presence of a minor population of CD3+CD16+ cells within peripheral blood lymphocytes (2.84%). Peripheral blood CD3+CD16+ lymphocytes consisted of all four subpopulations with respect to the expression of surface antigens CD4 and CD8汐; most were CD4-CD8汐+ (60.64%) and CD4-CD8汐-(36.77%). While the proportion of SLA- DR+ cells within both subpopulations was similar (8.01% of CD3+CD16+CD4-CD8汐+ lymphocytes and 7.41% of CD3+CD16+CD4- CD8汐- lymphocytes), the proportion of TCR-污汛+ cells was noticeably higher within CD3+CD16+CD4-CD8汐+ (43.48%) than CD3+CD16+CD4-CD8汐- (16.55%) lymphocytes. When the expression of individual surface antigens was analysed on peripheral blood CD3+CD16+ lymphocytes, most were CD8汐+ (62.44%), though some were also TCR- 污汛+ (32.56%), SLA-DR+ (7.55%) or CD4+ (2.59%). Expression of CD8汐 on CD3+CD16+ lymphocytes was not related to co-expression of other surface antigens, though most CD3+CD16+TCR-污汛+ lymphocytes (81.04%) and most CD3+CD16+CD4+ lymphocytes (69.50%) expressed CD8汐. Expression of SLA-DR was not related to the co-expression of TCR-污汛 or CD8汐, or to the co-expression of both antigens (TCR-污汛 and CD8汐) on CD3+CD16+CD4- lymphocytes. The results also showed the presence of peripheral blood lymphocytes with the combined phenotypeof T cells and NK cells in three-month old pigs. Though a functional analysis of the investigated cells was not performed in this study, future investigations should provide more insight about the functional properties of porcine peripheral blood CD3+CD16+ lymphocytes with distinct phenotypic characteristics, especially concerning antigen- specific responses and whether the results presented here are solely age-related
%K T cells
%K NK cells
%K swine
%K phenotype analysis
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=343070