%0 Journal Article
%T Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples
%A Konorov
%A Evgenii A.
%A Kurbakov
%A Konstantin A.
%A Kuznetsova
%A Oksana A.
%A Minaev
%A Mikhail Y.
%J -
%D 2019
%R 10.17113/ftb.57.01.19.5983
%X Sa£żetak Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair
%K real-time PCR
%K HRM
%K starter cultures
%K fermented sausages
%K Lactobacillus sakei
%K Lactobacillus curvatus
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=319530