%0 Journal Article
%T Faecal calprotectin determination: impact of preanalytical sample treatment and stool consistency on within- and between-method variability
%A Brencic
%A Tina
%A Honovic
%A Lorena
%A Juricic
%A Gordana
%A Njegovan
%A Milena
%A Tesija Kuna
%A Andrea
%J -
%D 2019
%R 10.11613/BM.2019.010707
%X Sa£¿etak Introduction: We assessed the differences in faecal calprotectin (FC) concentrations measured by two assays depending on the stool consistency and extraction method. Materials and methods: Stool samples were extracted using the EliA Stool Extraction Kit, Calex£¿ Cap extraction device and respective weighing methods, while FC concentrations were measured using the EliATM Calprotectin and B¨¹hlmann fCAL£¿ Turbo method and checked for within- and between-method variability with regard to extraction method and stool consistency category. Extraction yield was evaluated for impact of different sample incubation time (10 min and 1 h) in extraction buffer for both methods and for impact of different initial sample dilutions (1:50, 1:100, 1:500) for fCAL£¿ Turbo method. Results: Results determined from Calex£¿ Cap extracts were higher compared to weighing method extracts (mean bias 33.3%; P < 0.001), while no significant difference was found between results obtained with EliA Stool Extraction Kit and weighing method (mean bias 0.1%; P = 0.484), in both cases irrespective of stool consistency. B¨¹hlmann fCAL£¿ Turbo results were higher than EliATM Calprotectin results (mean bias 32.3%, P = 0.025 weighing method; and mean bias 53.9%, P < 0.001 extraction devices), the difference is dependent on stool consistency and FC concentration. Significantly higher FC extraction yield was obtained with longer sample incubation time for both methods (P = 0.019 EliATM Calprotectin; P < 0.001 fCAL£¿ Turbo) and with increasing initial sample dilution for fCAL£¿ Turbo method (P < 0.001). Conclusion: Preanalytical stool sample handling proved to be a crucial factor contributing to within- and between-FC assay variability. Standardization is urgently needed in order to assure comparable and reliable FC results
%K calprotectin
%K preanalytical phase
%K inflammatory bowel disease
%K immunoassay
%U https://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=317001