%0 Journal Article
%T Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in ¦Â-Amyloid-Induced Rats Model of Alzheimer¡¯s Disease
%A Abdul Malek
%A Siti Nur Aqilah
%A Ahmad Munawar
%A Munirah
%A Azmi
%A Norazrina
%A Haron
%A Hasnah
%A Ibrahim
%A Farah Wahida
%A Mohd Rosli
%A Nur Hasnieza
%A Ooi
%A Theng Choon
%A Rajab
%A Nor Fadilah
%A Rosli
%A Hanisah
%A Shahar
%A Suzana
%A Sharif
%A Razinah
%J -
%D 2020
%R https://doi.org/10.1155/2020/5126457
%X This study aimed to determine the effects of tropical fruit juice mixture (pomegranate, white guava, and Roselle) on biochemical, behavioral, and histopathological changes of ¦Â-amyloid- (A¦Â-) induced rats. Formulation 8 (F8) of tropical fruit juice mixture was chosen for this present study due to its high phenolic content and antioxidant capacity. Forty Wistar male rats were divided into five groups: dPBS (sham-operated control), dA¦Â (A¦Â control), JPBS (F8 and PBS), JA¦Â (F8 and A¦Â), and IBFA¦Â (ibuprofen and A¦Â). F8 (5£¿ml/kg BW), and ibuprofen (10£¿ml/kg BW) was given orally daily for four weeks before the intracerebroventricular infusion of A¦Â for two weeks. Histological analysis and neuronal count of hippocampus tissue in the Cornu Ammonis (CA1) region showed that supplementation with F8 was able to prevent A¦Â-induced tissue damage and neuronal shrinkage. However, no significant difference in locomotor activity and novel object recognition (NOR) percentage was detected among different groups at day 7 and day 14 following A¦Â infusion. Only effect of time differences (main effect of day) was observed at day 7 as compared to day 14, where reduction in locomotor activity and NOR percentage was observed in all groups, with F (1, 7)£¿=£¿6.940, and F (1, 7)£¿=£¿7.152, , respectively. Besides, the MDA level of the JA¦Â group was significantly lower ( ) than that of the dPBS group. However, no significant changes in SOD activity were detected among different groups. Significant reduction in plasma CRH level ( ) and iNOS expression ( ) in the brain was detected in the JA¦Â group as compared to the dA¦Â group. Hence, our current findings suggest that the tropical fruit juice mixture (F8) has the potential to protect the rats from A¦Â-induced neurotoxicity in brain hippocampus tissue possibly via its antioxidant properties and the suppression of iNOS expression and CRH production
%U https://www.hindawi.com/journals/ecam/2020/5126457/