%0 Journal Article
%T Polymorphism of Transcription Factor 7-Like 2 in Obese Patients with Type 2 Diabetes Mellitus
%A Defeng Kong
%A Xue Han
%A Hang Guo
%A Rui Xia
%J Journal of Biosciences and Medicines
%P 9-15
%@ 2327-509X
%D 2020
%I Scientific Research Publishing
%R 10.4236/jbm.2020.811002
%X <strong>Objective: </strong>The article is to investigate the relationship between the polymorphism of transcription factor 7-like 2 (TCF7L2) gene rs7903146 and type 2 diabetes mellitus with obesity in Chinese Han population. <strong>Methods: </strong>216 patients with type 2 diabetes mellitus (T2DM), 92 males, 124 females, 194 normal controls, 89 males and 105 females. The polymorphism of TCF7L2 gene rs7903146 was measured by PCR-RFLP. PCR amplifier was used for amplification reaction, followed by polymorphism analysis of TCF7L2 gene: the reaction system was 20 ¦Ìl, among which restriction endonuclease was 0.5 ¦Ìl and PCR product was 7.5 ¦Ìl. Restriction enzyme SspI (Fermentas) was used for PCR product and was bathed at 37<span style=\"white-space:nowrap;\">¡ã</span>C for 16 h. The enzyme digestion products were detected and genotypes were identified by 2% agarose gel electrophoresis. The genotyping was repeated in 10% of the samples, and the genotyping rate was 100%. Height and weight were measured and BMI calculated. According to BMI, the experimental group was further divided into the obese group (n = 137, BMI &gt; 25 kg/m<sup>2</sup>) and the normal body mass group (n = 79, BMI &lt; 25 kgm<sup>2</sup>). Normal control group: BMI &lt; 25 kgm<sup>2</sup>: FBG &lt; 6.1 mmol/L and 2 h BG &lt; 7.8 mmol/L after meal. Finally, SPSS18.0 software was used for analysis. <strong>Results:</strong> FBG, 2 h BG, FIN and Lg homa-ir showed significant differences between the control group and the T2DM groups (<em>P</em> &lt; 0.01), while no statistical difference was found between the other parameters (<em>P</em> &gt; 0.05). There was a significant difference in genotype frequency between the control group and the experimental group (<em>¦Ö</em><sup>2</sup> = 17.382, <em>P</em> &lt; 0.001), but no significant difference in allele frequency between the control group and the T2DM group (<em>P</em> &gt; 0.05). Genotype frequency at BMI level was significantly different between the control group and the T2DM group (<em>¦Ö</em><sup>2</sup> = 20.427, <em>P</em> = 0.001), but there was no significant difference in allele frequency distribution (<em>P</em> &gt; 0.05). The TT type and TC + CC type were significantly different between the T2DM group and the control group (<em>¦Ö</em><sup>2</sup> = 78.154, <em>P</em> = 0.006) and the T2DM obesity group and the control group (<em>¦Ö</em><sup>2</sup> = 7.247, <em>P</em> = 0.009), but there was no significant difference between the T2DM normal body mass group and the control group (<em>P</em> &gt; 0.05). In addition, there was no
%K Type 2 Diabetes Mellitus
%K Obese
%K TCF7L2
%K Polymorphism Study
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=103951