%0 Journal Article
%T Nucleosome remodeling at origins of global genome¨Cnucleotide excision repair occurs at the boundaries of higher-order chromatin structure
%A Mark Bennett
%A Matthew Leadbitter
%A Patrick van Eijk
%A Shirong Yu
%A Shuvro Prokash N
%A Yumin Teng
%A i
%J Genome Research
%D 2019
%R http://www.genome.org/cgi/doi/10.1101/gr.237198.118
%X Abstract Repair of UV-induced DNA damage requires chromatin remodeling. How repair is initiated in chromatin remains largely unknown. We recently demonstrated that global genome¨Cnucleotide excision repair (GG-NER) in chromatin is organized into domains in relation to open reading frames. Here, we define these domains, identifying the genomic locations from which repair is initiated. By examining DNA damage¨Cinduced changes in the linear structure of nucleosomes at these sites, we demonstrate how chromatin remodeling is initiated during GG-NER. In undamaged cells, we show that the GG-NER complex occupies chromatin, establishing the nucleosome structure at these genomic locations, which we refer to as GG-NER complex binding sites (GCBSs). We demonstrate that these sites are frequently located at genomic boundaries that delineate chromosomally interacting domains (CIDs). These boundaries define domains of higher-order nucleosome¨Cnucleosome interaction. We demonstrate that initiation of GG-NER in chromatin is accompanied by the disruption of dynamic nucleosomes that flank GCBSs by the GG-NER complex
%U https://genome.cshlp.org/content/29/1/74.abstract