%0 Journal Article
%T Inhibitory effects of CP on the growth of human gastric adenocarcinoma BGC
%A Ai-Ying Li
%A Bao-Jun Zhou
%A Bin Yue
%A Hai-Jun Wang
%A Li-Qiao Fan
%A Ran An
%A Yong Li
%A Yu Liu
%A Zhan-Xue Zhang
%J Journal of International Medical Research
%@ 1473-2300
%D 2018
%R 10.1177/0300060518761505
%X To investigate the potential antitumour effects of [2-(6-amino-purine-9-yl)-1-hydroxy-phosphine acyl ethyl] phosphonic acid (CP) against gastric adenocarcinoma. Human BGC-823 xenotransplants were established in nude mice. Animals were randomly divided into control and CP groups, which were administered NaHCO3 vehicle alone or CP dissolved in NaHCO3 (200 ¦Ìg/kg body weight) daily, respectively. Tumour volume was measured weekly for 6 weeks. Resected tumours were assayed for proliferative activity with anti-Ki-67 or anti-proliferating cell nuclear antigen (PCNA) antibodies. Cell apoptosis was examined using terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assays and with caspase-3 immunostaining. Proteins were measured by Western blotting. There was a significant reduction in tumour volume and a reduced percentage of Ki-67-positive or PCNA-positive cells in the CP group compared with the control group. The percentage of TUNEL-positive or caspase 3-positive cells significantly increased following CP treatment compared with the control group. Tumours from the CP group had higher levels of phosphorylated-extracellular signal-regulated kinase (p-ERK) and phosphorylated-AKT (p-AKT) compared with control tumours. CP treatment inhibited tumour growth and induced tumour cell apoptosis in a nude mouse model of BGC-823 gastric adenocarcinoma. Activation of the AKT and ERK signalling pathways may mediate this antitumour activity
%K CP
%K gastric carcinoma
%K BGC-823 cells
%K proliferation
%K apoptosis
%U https://journals.sagepub.com/doi/full/10.1177/0300060518761505