%0 Journal Article
%T Coagulation phenotype of wild
%A Alexander Bauer
%A Alexandra Kopiˋ
%A Helmut Glantschnig
%A Karima Benamara
%A Maria Schuster
%A Peter Leidenm邦hler
%A Werner Hˋllriegl
%J Laboratory Animals
%@ 1758-1117
%D 2019
%R 10.1177/0023677218811059
%X Genetically engineered mouse models are used to investigate beneficial treatment in haemophilia by comparison with wild-type mice. It has been recognized that wild-type and haemophilic mice of different genetic backgrounds show different bleeding phenotypes. We assessed ex-vivo coagulation parameters in nine wild-type substrains of 129S1/Sv, BALB/c and C57BL/6 mice applying thromboelastography (TEG), activated partial thromboplastin time (aPTT), prothrombin time (PT) and fibrinogen levels. The comprehensive ex-vivo data are discussed in view of results from a tail-tip bleeding assay. Time to first clot formation (R-time) showed higher within-substrain (CV range: 28每54%) and higher between-substrain (median range: 25.53每42.60ˋmin) variation for BALB/c than for C57BL/6 mice (CV range: 14每31%; median range: 22.45每24.93ˋmin). Median R-time for 129S1/Sv mice was 30.42ˋmin (CV: 33%). No distinct strain differences were observed for maximum amplitude (MA), aPTT, or PT, but males generally showed higher MA and shorter aPTT than females. Males of all substrains had higher fibrinogen levels than females. The heightened in-vivo variability (CV range: 81每171%; median range: 36.00每469.50ˋmg) in the tail-tip bleeding assay and increased blood loss in wild-type C57BL/6 male mice was not reflected in ex-vivo coagulation parameters. In general, ex-vivo coagulation results appeared consistent within substrains, but showed substrain and sex differences of variable magnitudes. We conclude that alignment of the mouse substrain genetic background to the experimental model is critical to reduce data variability and animal numbers
%K thromboelastography
%K coagulation parameters
%K reduction
%K strain differences
%K genetic background
%U https://journals.sagepub.com/doi/full/10.1177/0023677218811059