%0 Journal Article
%T In vitro evaluation of copper oxide nanoparticle
%A Anreddy Rama Narsimha Reddy
%A Srividya Lonkala
%J Toxicology and Industrial Health
%@ 1477-0393
%D 2019
%R 10.1177/0748233718819371
%X This study aimed to evaluate the in vitro cytotoxicity and oxidative stress induced by the copper oxide nanoparticles (CuO NPs) in human embryonic kidney cell line (HEK-293) cells following exposure. CuO NPs size <50 nm were used in this study. HEK-293 cell cultures were exposed to different concentrations of CuO NPs between 3 ¦Ìg/ml and 300 ¦Ìg/ml and quartz (known as cytotoxic agent) and assessed for cell viability-mitochondrial function (MTT assay), cell membrane damage (lactate dehydrogenase (LDH) assay), reduced glutathione (GSH), interleukin-8 (IL-8), and lipid peroxidation levels. The IC50 value of NPs was found to be 65.5 ¦Ìg/ml. Exposure of HEK cells to CuO NPs (10¨C300 ¦Ìg/ml) resulted in concentration-dependent cell membrane damage, increased production of IL-8, increased thiobarbituric acid reactive substance (TBARS), and decreased intracellular GSH levels. The significant increases in IL-8, TBARS, and LDH levels along with decreased GSH levels indicated induction of oxidative stress in cells. Our preliminary data suggest that oxidative stress might contribute to CuO NPs-induced cytotoxicity in HEK-293 cells
%K Nanoparticles
%K copper oxide
%K copper oxide nanoparticles
%K human embryonic kidney-293 cells
%K oxidative stress
%K cytotoxicity
%K in vitro
%U https://journals.sagepub.com/doi/full/10.1177/0748233718819371