%0 Journal Article
%T Evaluation and validation of an analytical approach for high
%A Alain Paris
%A Douglas N Rutledge
%A Estelle Rathahao-Paris
%A Jean-Claude Tabet
%A Nawel Boussaid
%A Nicole Picard-Hagen
%A Pierre-Louis Toutain
%A Sandra Alves
%A V谷ronique Gayrard
%J European Journal of Mass Spectrometry
%@ 1751-6838
%D 2019
%R 10.1177/1469066718806450
%X Direct injection每mass spectrometry can be used to perform high-throughput metabolomic fingerprinting. This work aims to evaluate a global analytical workflow in terms of sample preparation (urine sample dilution), high-resolution detection (quality of generated data based on criteria such as mass measurement accuracy and detection sensitivity) and data analysis using dedicated bioinformatics tools. Investigation was performed on a large number of biological samples collected from sheep infected or not with scrapie. Direct injection每mass spectrometry approach is usually affected by matrix effects, eventually hampering detection of some relevant biomarkers. Reference compounds were spiked in biological samples to help evaluate the quality of direct injection每mass spectrometry data produced by Fourier Transform mass spectrometry. Despite the potential of high-resolution detection, some drawbacks still remain. The most critical is the presence of matrix effects, which could be minimized by optimizing the sample dilution factor. The data quality in terms of mass measurement accuracy and reproducible intensity was evaluated. Good repeatability was obtained for the chosen dilution factor (i.e., 2000). More than 150 analyses were performed in less than 16 hours using the optimized direct injection每mass spectrometry approach. Discrimination of different status of sheeps in relation to scrapie infection (i.e., scrapie-affected, preclinical scrapie or healthy) was obtained from the application of Shrinkage Discriminant Analysis to the direct injection每mass spectrometry data. The most relevant variables related to this discrimination were selected and annotated. This study demonstrated that the choice of appropriated dilution faction is indispensable for producing quality and informative direct injection每mass spectrometry data. Successful application of direct injection每mass spectrometry approach for high throughput analysis of a large number of biological samples constitutes the proof of the concept
%K High-throughput metabolomics
%K direct injection每mass spectrometry
%K high-resolution mass spectrometry
%K matrix effects
%K data analysis
%U https://journals.sagepub.com/doi/full/10.1177/1469066718806450