%0 Journal Article
%T Development of a High
%A Barton E. Slatko
%A Bo Wu
%A Catherine Bardelle
%A Darren Plant
%A David Murray
%A Eileen McCall
%A Helen Plant
%A Kelly L. Johnston
%A Kirsty Rich
%A Lindsey Cantin
%A Louise Ford
%A Mark J. Taylor
%A Mark Wigglesworth
%A Matthew Collier
%A Paul Harper
%A Rachel H. Clare
%A Roger Clark
%A Stephen A. Ward
%J SLAS DISCOVERY: Advancing Life Sciences R&D
%@ 2472-5560
%D 2019
%R 10.1177/2472555219838341
%X The Anti-Wolbachia (AĦ¤WOL) consortium at the Liverpool School of Tropical Medicine (LSTM) has partnered with the Global High-Throughput Screening (HTS) Centre at AstraZeneca to create the first anthelmintic HTS for neglected tropical diseases (NTDs). The AĦ¤WOL consortium aims to identify novel macrofilaricidal drugs targeting the essential bacterial symbiont (Wolbachia) of the filarial nematodes causing onchocerciasis and lymphatic filariasis. Working in collaboration, we have validated a robust high-throughput assay capable of identifying compounds that selectively kill Wolbachia over the host insect cell. We describe the development and validation process of this complex, phenotypic high-throughput assay and provide an overview of the primary outputs from screening the AstraZeneca library of 1.3 million compounds
%K high-throughput screening
%K phenotypic screening
%K neglected tropical diseases
%K Wolbachia
%K acumen
%K onchocerciasis
%K lymphatic filariasis
%U https://journals.sagepub.com/doi/full/10.1177/2472555219838341