%0 Journal Article
%T TSA
%A Bin Tean Teh
%A Ley-Moy Ng
%A Po-Hsien Lee
%A Xi Xiao Huang
%J SLAS DISCOVERY: Advancing Life Sciences R&D
%@ 2472-5560
%D 2019
%R 10.1177/2472555218823547
%X Thermal shift assay (TSA) is an increasingly popular technique used for identifying protein stabilizing conditions or interacting ligands in X-ray crystallography and drug discovery applications. Although the setting up and running of TSA reactions is a relatively simple process, the subsequent analysis of TSA data, especially in high-throughput format, requires substantial amount of effort if conducted manually. We therefore developed the Thermal Shift Assay¨CCurve Rapid and Automatic Fitting Tool (TSA-CRAFT), a freely available software that enable automatic analysis of TSA data of any throughput. TSA-CRAFT directly reads real-time PCR instrument data files and displays the analyzed results in a web browser. This software features streamlined data processing and Boltzmann equation fitting, which is demonstrated in this study to provide more accurate data analysis than the commonly used first-derivative method. TSA-CRAFT is freely available as a cross-operating system-compatible standalone tool (https://sourceforge.net/projects/tsa-craft/) and also as a freely accessible web server (http://tbtlab.org/tsacraft.html)
%K thermal shift assay
%K thermofluor
%K differential scanning fluorimetry
%K automatic Tm analysis
%K high-throughput ligand screening
%U https://journals.sagepub.com/doi/full/10.1177/2472555218823547